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Table 2 Sequences of the primers used in real-time RT-PCR reaction

From: Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

Primer name

Sequence (5′ to 3′)

Accession number

Forward

Reverse

GAPDH

GGTGGTGCTAAGCGTGTTA

CCCTCCACAATGCCAA

NM_204305

TRIM25

TACAACCACCACCCTCA

GATGCCAATGCCACAG

NM_001318458.1

MDA5

TGAAAGCCTTGCAGATGACTTA

GCTGTTTCAAATCCTCCGTTAC

AB371640.1

MAVS

CACCCACGAGGTCCATGTG

TGCTTCATCTGGGACATCATTG

NM_001012893.1

IFN-β

TCCAGCTCCTTCAGAATACG

TGCGGTCAATCCAGTGTT

NM_001024836

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Veterinary Research

ISSN: 1297-9716