Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

Figure 4

Knockdown of chicken TRIM25 can promote the replication of ALV-A. The expression of chicken TRIM25 in the DF1 cells transfected with the TRIM25-siRNA and NC-siRNA were detected by QPCR (A) and western blot (B) (the grey signals were analyzed using Image J software and the relative ratios were statistically calculated, **, P < 0.01). The intracellular proliferation of ALV-A virus after TRIM25-siRNA transfection was detected by immunofluorescence at 3 dpi (C). The effects of TRIM25-siRNA on the antigen titers of ALV-A in DF1 cells at different days post inoculation with the ALV-A-SDAU09C1 strain were detected by ELISA (D). Different letters on the bars indicate statistically significant differences (P < 0.05) and the same letter indicates no significant difference (P > 0.05) between the groups; dpi, days post inoculation.

Back to article page