Table 1 Primers for construction of pPICZα A-Nbs-pFc plasmids and detection of PRRSV ORF7 and cytokines
Gene | Primer | Sequence (5′–3′) |
|---|---|---|
Nbs-pFc | Forward | GGTGAATTCCAGGTCCAACTGCAGGAGTC |
Reverse | GGGTCTAGATCACTTGCCCTGTGTCTTGC | |
PRRSV ORF7 | Forward | AGATCATCGCCCAACAAAAC |
Reverse | GACACAATTGCCGCTCACTA | |
PAM IL-1β | Forward | CCTTGAAACGTGCAATGATGACT |
Reverse | GTGGAGAGCCTTCAGCATGT | |
PAM IL-6 | Forward | CCGGTCTTGTGGAGTTTCAG |
Reverse | CAGGGTCTGGATCAGTGCTT | |
PAM IL-8 | Forward | CCAGCATTCACAAGTCTTCTTGC |
Reverse | ATGTCCTCAAGGTAGGATGGGG | |
PAM IL-10 | Forward | GCATCCACTTCCCAACCA |
Reverse | TCGGCATTACGTCTTCCAG | |
PAM IL-12p40 | Forward | GTTTCAGACCCGACGAACTC |
Reverse | GAGGACCACCATTTCTCCAG | |
PAM IFN-α | Forward | ACTTCCACAGACTCACCCTCTATC |
Reverse | ATGACTTCTGCCCTGATGATCT | |
PAM IFN-β | Forward | TGCAACCACCACAATTCC |
Reverse | CTGAGAATGCCGAAGATCTG | |
PAM TNF-α | Forward | AGCCGCATCGCCGTCTCCTAC |
Reverse | CCTGCCCAGATTCAGCAAAGTCC | |
GAPDH | Forward | CCTTCCGTGTCCCTACTGCCAAC |
Reverse | GACGCCTGCTTCACCACCTTCT |