Figure 6
HP-PRRSV GD-HD replication by Nb6-pFc in PAM. Nbs-pFc and PRRSV Nsp9 were detected at A 24 hpi and D 36 hpi by Western blot using HRP-goat anti-swine IgG and anti-PRRSV N protein mAb, respectively. Relative levels of PRRSV RNA at B 24 hpi and E 36 hpi detected by RT-qPCR using PRRSV ORF7-specific primers. The GAPDH mRNA level served as an internal reference. Progeny virus released in the cell medium was measured by TCID50 at C 24 hpi and F 36 hpi. Data are expressed as means from three independent experiments. P values were calculated using ANOVA as < 0.05 (*), < 0.01 (**), and < 0.001 (***) compared with cells infected with PRRSV alone (ns: not significant).