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Figure 1 | Veterinary Research

Figure 1

From: Nanobody Nb6 fused with porcine IgG Fc as the delivering tag to inhibit porcine reproductive and respiratory syndrome virus replication in porcine alveolar macrophages

Figure 1

Construction and analysis of purified pig Fc linked nanobodies. A Design and construction of pPICZα-A-Nbs-pFc. B Speculative structural model of Nbs-pFc. C SDS-PAGE identification of purified Nbs-pFc. The predicted sizes of the pFc linked nanobodies are 80 kDa in absence of β-mercaptoethanol and 40 kDa in presence of β-mercaptoethanol. M, protein marker; lanes 1 to 4, reduced Nb6-pFc, non-reduced Nb6-pFc, reduced Nb53-pFc, non-reduced Nb53-pFc, respectively. D Western blot identification of purified pig Fc-linked nanobodies by HRP-conjugated goat anti-swine IgG and rabbit anti-camel sera.

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Veterinary Research

ISSN: 1297-9716