Table 1 List of oligonucleotides used in this study
Name | Sequence (5′–3′) | Product (bp) | Annealing T (°C) | Application |
|---|---|---|---|---|
SNUC-F | ACAATTCGTTCAAGCCGAG | 513 | 58 | Colony PCR confirmation |
SNUC-R | AATGTAATTCAGCTCCGCC | |||
pmET32c-F | CATGCATTGACAATTAATCATC | 110 | 52 | qPCR to evaluate the plasmid (MN049497.1) content of BGs |
pmET32c-R | ACCTCCTCTCCTTCTTAA | |||
ACTB-F | CGTGACATCAAGGAGAAG | RT-qPCR for β-actin (NM_205518) | ||
ACTB-R | AGGACTCCATACCCAAGAAAGATG | 187 | 57 | |
Probe | FAM-CACCTGAACCTCTCATTGCCAA-BHQ1 | |||
IL1b-F | CTTCGACATCAACCAGAA | RT-qPCR for IL1b (NM_204524) | ||
IL1b-R | CGACATGTAGAGCTTGTA | 196 | 55 | |
Probe | FAM-TGCTTCGTGCTGGAGTCACC-BHQ1 | |||
IL6-F | CTGGAATTCATTCAAGAGA | RT-qPCR for IL6 (NM_204628) | ||
IL6-R | CATCGGGATTTATCACCATCTG | 112 | 57 | |
Probe | FAM-AACGTCGAGTCTCTGTGCTACA-BHQ1 | |||
TNFSF15-F | GAGCACACCTGACAGTGAAGAA | RT-qPCR for TNFSF15 (NM_001024578) | ||
TNFSF15-R | CTCGGAAAGTGACCTGAGCAT | 183 | 64 | |
Probe | FAM-CCTCCAGCACCACGGGAAGCCATCTG-BHQ1 |