Figure 3

Up-regulation of DUSP6 by IBV infection. A Northern blotting analysis of DUSP6 in IBV-infected Vero and H1299 cells. Vero and H1299 cells were infected with IBV at MOI of 1 and harvested at the indicated time points. Total RNA were extracted and were separated on agarose gel, followed with northern blotting analysis by using specific DIG-labeled DUSP6 probe. B Vero, H1299 and DF-1 cells were infected with IBV or mock infection and harvested at the indicated time points. Cell lysates were subjected to Western blot analysis by using anti-DUSP6 antibody. β-tubulin was detected as the loading control. The experiments were repeated in triplicate and the representative figures are shown. The signal of protein bands was determined by Image J software. The intensities of DUSP6 were normalized to total β-tubulin. The ratio of DUSP6 in IBV infected cells to mock infected cells are shown as DUSP6 (+ :−).