Figure 1

Infection with IBV results in activation of ERK signaling in Vero, H1299 and DF-1 cells. Vero, H1299, and DF-1 cells were infected with IBV-Beaudette at MOI of 1 or mock infection. At the indicated hours post-infection (hpi), cells were collected and lysed for Western blot analysis to detect phospho-ERK1/2 (p-ERK1/2), ERK1/2, and IBV N protein. The experiments were repeated in triplicate and the representative figures are shown. The signal of protein bands was determined by Image J. The intensities of p-ERK1/2 or p-ERK2 were normalized to total ERK1/2 or total ERK2. The ratio of p-ERK in IBV infected cells to mock infected cells are shown as p-ERK1/2 (+ :−) or p-ERK2 (+ :−).