Evaluation of phenotypic profile of mature DCs in response to rEaGAPDH. Double-positive cell-surface markers in chicken DCs after incubated with rEaGAPDH, LPS, pET-32a protein or PBS were analysed by flow cytometry. A Dot plot percentage of MHCII/CD1.1, MHCII/CD11c, MHCII/CD80 and MHCII/CD86. Bar graphs showing percentage of B MHCII/CD1.1, C MHCII/CD11c, D MHCII/CD80 and E MHCII/CD86. Results presented here are from an independent experiment that is representative of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001).