Cannabis-derived cannabidiol and nanoselenium improve gut barrier function and affect bacterial enzyme activity in chickens subjected to C. perfringens challenge

Table 1 Genes and primers used in the study.

Gene	Primer	Sequence (5′-3′)	Melting temperature (°C)	Product size (nt)	GenBank accession no.
ACTB	Forward	CGGACTGTTACCAACACCCA	58	115	NM_205518
ACTB	Reverse	TCCTGAGTCAAGCGCCAAAA		115	NM_205518
GAPDH	Forward	GCACGCCATCACTATCTT	58	82	NM_204305
GAPDH	Reverse	GGACTCCACAACATACTCAG		82	NM_204305
ZO-1	Forward	TCGCTGGTGGCAATGATGTT	58	89	XM_413773
ZO-1	Reverse	TTGGTCTCCTTCCTCTAATCCTTCTT		89	XM_413773
GLP2	Forward	TGTGTTCAGACGGTAAGG	58	127	NM_001163248
GLP2	Reverse	TCATCCAGTGCCATCTTC		127	NM_001163248
HSP70	Forward	GGCAATAAGCGAGCAGTG	58	146	NM_001006685
HSP70	Reverse	CGAGTGATGGAGGTGTAGAA		146	NM_001006685
TFF2	Forward	ACTACCCTACTGAGAGAACAAA	58	143	XM_416743
	Reverse	CTGAAGAACCTGCTCAACTG
TLR4	Forward	CAAGCACCAGATAGCAACA	58	146	FJ915527
	Reverse	CACTACACTACTGACAGAACAC
JAM2	Forward	TCCTCCCACTACTCCAATATG	58	134	XM_026849998
	Reverse	ACTGCCTGTTCCTGTCTT

ACTB β-actin, GAPDH glyceraldehyde-3-phosphate dehydrogenase, ZO-1 Zonula occludens-1, GLP2 Glucagon-like peptide-2, HSP70 Heat Shock Protein 70, TFF2 Trefoil Factor 2, TLR4 Toll-like Receptor 4, JAM2 Junctional Adhesion Molecule 2.

ISSN: 1297-9716