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Table 2 Gene-specific primers flanked by T7 RNA polymerase promoter sequences

From: RNAi-mediated silencing of Trichinella spiralis serpin-type serine protease inhibitors results in a reduction in larval infectivity

Primer

Sense (5′–3′)

TsSPI dsRNA-566 F

GGTCGTTCGTTTCATCGC

TsSPI dsRNA-566 R

AGCACCGCTAACTTTGGA

TsSPI dsRNA-566 T7 F

TAATACGACTCACTATAGGG GGTCGTTCGTTTCATCGC

TsSPI dsRNA-566 T7 R

TAATACGACTCACTATAGGG AGCACCGCTAACTTTGGA

dsGFP F

TCCTGGTCGAGCTGGACGG

dsGFP T7 F

TAATACGACTCACTATAGGG TCCTGGTCGAGCTGGACGG

dsGFP R

CGCTTCTCGTTGGGGTCTTTG

dsGFP T7 R

TAATACGACTCACTATAGGG CGCTTCTCGTTGGGGTCTTTG

  1. The underlined portion is the T7 promoter sequence
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Veterinary Research

ISSN: 1297-9716