Gene specificity of RNAi induced by dsRNA–TsSPI. A qPCR analysis of relative transcription levels of the TsKaSPI gene in larvae after incubation for 3 days in dsRNA–TsSPI. B Western blot analysis of anti-TsSPI- and anti-TsKaSPI- specific antibodies of relative TsSPI and TsKaSPI protein expression levels after incubation in dsRNA–TsSPI for 3 days. Assays were performed in triplicate, and the data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ΔP > 0.05 compared with the PBS group.