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Figure 8 | Veterinary Research

Figure 8

From: Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1

Figure 8

pUL47 interacted with STAT1 in the cytoplasm and inhibited ISG expression induced by STAT1. A HEK293T cells were coexpressed pcaggs-STAT1-myc with pcaggs-UL47-Flag, pcaggs-STAT1-myc with an empty vector and pcaggs-UL47-Flag with an empty vector (magnification: 200×; scale bar: 10 μm). B BHK21 cells contained pcDNA3.1-C-UL47-LgBiT coexpressed with pcDNA3.1-N-STAT1-SmBiT, FKBP-SmBiT coexpressed with FRB-LgBiT as positive controls, Halotag-SmBiT coexpressed with pcDNA3.1-C-UL47-LgBiT, pcDNA3.1-C-UL47-LgBiT coexpressed with FKBP-SmBiT and pcDNA3.1-N-STAT1-SmBiT coexpressed with FRB-LgBiT as negative controls. The luciferase signal was detected via NanoBiT after 20-h posttransfection. C DEV UL47 inhibited the mRNA levels of Mx and OASL induced by STAT1. DEF cells were co-transfected with pcaggs-STAT1-myc and pcaggs-UL47-Flag or an empty vector. The cells were harvested and detected by RT-qPCR at 36 h posttransfection. Protein expression was confirmed by Western blotting. All experiments were performed in triplicate (n = 3). The data was analyzed by one-way ANOVA. *P < 0.05, **P < 0.01 and ****P < 0.0001.

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Veterinary Research

ISSN: 1297-9716