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Figure 1 | Veterinary Research

Figure 1

From: Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1

Figure 1

Purified fusion protein and western blot analysis. A Lane 1, pET32a-UL47, non-induced; Lane 2, pET-32a-UL47 recombinant bacteria after induction in E. coli BL21 (DE3); Lane 3, pET-32a(+) vector; Lane 4, pET-32a-UL47 recombinant bacterial supernatant; Lane 5, pET-32a-UL47 recombinant bacterial sediment. B Purification of the recombinant protein. Lane 1, purified protein (approximately 55 kDa); C Expressed protein was recognized with rabbit anti-DEV antibody (1:800). Lane 1, recombinant protein (approximately 55 kDa); Lane 2, pET-32a (+) vector; M. Precision Plus Protein™ Dual Color Standards.

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