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Table 1 Characteristics of the Wap65-2 peptides identified by mass spectrometry MALDI-TOF/TOF

From: Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation

Protein accession, description

Sequence coverage

Mascot score

Precursor mass

Peptide score

Peptide sequence

Observed

Theoretical

Wap65-2a

 XP_018924890

60

692

1392.6594

1391.6456

51

K.DIEFDAITPDEK.G

 PREDICTED: hemopexin-like [Cyprinus carpio]

  

860.4074

859.4229

61

K.GNTFFFK.G

1480.7651

1479.7358

39

K.GFSGPAELSNNIFK.E

1870.9130

1869.9010

138

K.ELDDYHLLGHVDAAFR.M

2496.1309

2495.0965

163

R.MHHQDDPSVHDHIYFFLDDK.V

2825.2896

2824.3378

165

K.GYPVEIQQEFPDVPSHLDAAVECPK.G

1415.7103

1414.6803

38

K.GECITDSVLFFK.G

1185.5932

1184.5713

64

K.GNEIYSFDIK.T

1422.6446

1421.6285

66

R.SYAFQDEMYIR.L

1152.5800

1151.5472

63

R.DGSHHFPISR.L

1816.0287

1814.9930

43

K.SAVHYTLIEGYPKPLK.E

1265.6452

1264.6122

78

K.MYDIDLAATPR.A

1558.7466

1557.7511

89

K.VWAHLPNCTSAFR.Wa

2093.8218

2091.9050

114

R.WLEHYYCFHGYNFTR.Fa

Wap65-2b

 XP_018924890

58

752

2292.9663

2290.9700

123

K.DAAEGHSHPNGEDHHDAKPDR.C

 PREDICTED: hemopexin-like [Cyprinus carpio]

  

1392.6594

1391.6456

51

K.DIEFDAITPDEK.G

860.4074

859.4229

61

K.GNTFFFK.G

1480.7651

1479.7358

39

K.GFSGPAELSNNIFK.E

1870.9130

1869.9010

138

K.ELDDYHLLGHVDAAFR.M

2496.1309

2495.0965

163

R.MHHQDDPSVHDHIYFFLDDK.V

2825.2896

2824.3378

165

K.GYPVEIQQEFPDVPSHLDAAVECPK.G

1415.7103

1414.6803

38

K.GECITDSVLFFK.G

1185.5932

1184.5713

64

K.GNEIYSFDIK.T

1422.6446

1421.6285

66

R.SYAFQDEMYIR.L

1152.5800

1151.5472

63

R.DGSHHFPISR.L

1816.0287

1814.9930

43

K.SAVHYTLIEGYPKPLK.E

1265.6452

1264.6122

78

K.MYDIDLAATPR.A

1558.7466

1557.7511

46

K.VWAHLPNCTSAFR.W

2093.8218

2091.9050

114

R.WLEHYYCFHGYNFTR.Fa

  1. aPeptides identified after deglycosylation, N–N-linked glycosylation sites.