Figure 2

cDNA and deduced amino acid sequence of carp Wap65-2 (A) and predicted tertiary structure of carp Wap65-2 with rabbit hemopexin (B). The signal peptide sequence is shaded in dark grey and the six hemopexin-like repeats in the sequence are shaded in light grey. The inverted dark triangles indicate metal ion binding sites. Potential N-linked glycosylation sites are boxed, and predicted phosphorylation sites are denoted with stars. Peptides identified by mass spectrometry are underlined. Peptides identified after N-glycosylation are double underlined in red. Wap65-2 is shown as a colored cartoon, while the structural analog (rabbit hemopexin) is displayed using a backbone trace.