Expression of immune-related genes and 16S rRNA in carp tissue after bacterial challenge with A. salmonicida. IL-1βl—interleukin 1β (A), iNOS—inducible nitric oxide synthase (B). Quantification of A. salmonicida in tissues using 16S rRNA gene expression (C) (n = 6). The results are presented as normalized copies per 100 000 copies of reference genes 40S ribosomal protein S11 and elongation factor 1 alpha. The bars represent means ± standard deviation. Stars indicate significant differences between the control and treated group (A, B) while different letters indicate differences between tissues (C).