Figure 1

Validation of expression profiling data by RT-qPCR. A The diagram of the operon encoding ZnuACB transporter. B DEGs were validated by RT-qPCR. The relative gene expression level was calculated using the 2^−ΔΔCt value method and normalized by the gapA housekeeping gene. * significant at p < 0.05, ** significant at p < 0.01 and *** significant at p < 0.001. Data presented as mean ± standard deviations of three independent experiments. C Comparison of 7 DEG between RNA-Seq and RT-qPCR data. The y axes represent the log2 (fold change) measured by RNA-Seq and RT-qPCR, respectively.