Figure 3

A GCN was constructed following PRRSV infection, indicating three substantial core groups (3 dpi, lungs, and BLNs). Each node (gene) colour is separated according to the associated immunity-responsible tissues: lungs (red), BLNs (green), and tonsils (blue). Nodes corresponding to each tissue were colour-coded using a gradient, based on each time point. The PCIT algorithm produced significant connections, and a titular threshold (\({r}^{2}\)>0.90) was used for the network analysis. The entire GCN contained 630 nodes connected by 7565 edges. The node size was mapped to the highest log₂ FC value among all time points and all tissues. The edge widths represent the correlation coefficient between nodes, and the edge colours indicate whether the correlation coefficient had a positive (grey) or negative (black) value. Gene-clustering analysis using the k-means clustering algorithm in the MultiExperiment Viewer software revealed five clear expression patterns at different time points (left to right) and in different tissues (same colour as the node). The y axes in the graphs showing the clustering analysis represent the log₂ FC values. The number of genes and the proportions of each cluster are shown in the top left corner.