Figure 8

The effect of CATH-B1 on DNA-induced nitric oxide production in HD11 cells. HD11 cells were stimulated with 5 nM ODN-2006 for 20 h in the presence or absence of different concentrations of cathelicidins. A The amount of NO in the cell supernatant were measured by Griess assay. Data are represented as mean  ±  SEM of three independent experiments of triplicate samples per experiment. **P ≤ 0.01; ***P ≤ 0.005; ****P ≤ 0.001 compared to the no peptide (0 µM) sample. B ITC thermogram of CATH-B1 binding ODN-2006. ITC analysis data were calculated as Kd = 6.4 × 10⁻⁸ M, ΔH = − 65.5 kJ/mol and ΔS = − 73.5 J/mol.