Figure 2

The effect of CATH-B1 on bacterial phagocytosis in chicken macrophages. Cells were infected with APEC for 3 h in the presence of 0–10 μM CATH-B1 (HD11 cells) or 5 μM CATH-B1 (primary macrophages), then gentamicin was added to kill extracellular bacteria for 1 h. Finally, cells were lysed to quantify intracellular bacteria in A HD11 cells and B in primary macrophages. Data are represented as mean  ±  SEM of three independent experiments of triplicate samples per experiment. *P ≤ 0.05; **P ≤ 0.01.