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Figure 10 | Veterinary Research

Figure 10

From: Molecular characterization of a Trichinella spiralis serine proteinase

Figure 10

rTsSerp facilitation or anti-rTsSerp serum suppression of larval invasion of IECs. Trichinella spiralis MLs were first activated into IILs using 5% swine bile for 2 h at 37 °C, the IILs were added onto the IEC monolayer, and the IILs that invaded the IECs were observed under microscopy after culture for 2 h (400 ×). A Larvae invading the IEC monolayer were mobile and migratory. B Non-invaded IILs coiled on the surface of the IEC monolayer. C Non-invaded IILs coiled on the surface of C2C12 cells, which were not sensitive to larval invasion and utilized as negative control cells. D and E rTsSerp facilitation of IIL invasion of IECs. F Anti-rTsSerp serum suppression of IIL invasion of IECs. *P < 0.01 compared to TRX-tag, anti-TRX-tag serum and pre-immune serum. Scale bars: 100 μm.

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