Recombinant plasmid | Sense primer (5′ → 3′) | Anti-sense primer (5′ → 3′) | Restriction sitesa |
---|---|---|---|
pEGFP-M | TCGGAATTCAATGGACTCATCCAGGAC | GCAGTCGACTTATTTCCTGAAAGG | EcoRI/SalI |
pCMV-HA-M | TTGAATTCGGATGGACTCATCCAGGAC | GCACTCGAGTTATTTCCTGAAAGG | EcoRI/XhoI |
pGEX-6p-M | TGCGAATTCATGGACTCATCCAGGAC | GGCACTCGAGTTATTTCCTGAAAGG | EcoRI/XhoI |
pDsRed-chBRD2 | CAGCTCGAGCTATGCTGCAGAATGTGAATCC | TGGGATCCTTAACCCGAATCCGAGTCGCT | XhoI/BamHI |
pCMV-Myc-chBRD2 | AGGAATTCAAATGCTGCAGAATGTGAATCC | TGCTCGAGTTAACCCGAATCCGAGTCGC | EcoRI/XhoI |
pET-32a-chBRD2 | CAGGAATTCATGCTGCAGAATGTGAATCC | TGCTCGAGTTAACCCGAATCCGAGTCGC | EcoRI/XhoI |
pCMV-HA-M(1–178) | TTGAATTCGGATGGACTCATCCAGGAC | GACTCGAGTTACAAGGAGACAAAATTCAC | EcoRI/XhoI |
pCMV-HA-M(1–245) | TTGAATTCGGATGGACTCATCCAGGAC | AACTCGAGTTATACAGTGGACATAAGCCC | EcoRI/XhoI |
pCMV-HA-M(1–263) | TTGAATTCGGATGGACTCATCCAGGAC | GACTCGAGTTATCTCCTTATCTTTTCCTC | EcoRI/XhoI |
pCMV-HA-M(264–364) | GAGAATTCGGCTCAATCTATCTGTTGG | GCACTCGAGTTATTTCCTGAAAGG | EcoRI/XhoI |
pCMV-HA-M(314–364) | GAGAATTCGGATACTCTGGAGCCAGACC | GCACTCGAGTTATTTCCTGAAAGG | EcoRI/XhoI |
pCMV-HA-M(264–313) | GAGAATTCGGCTCAATCTATCTGTTGGG | CTCTCGAGTTACTTGGCAACCTGGGGAGA | EcoRI/XhoI |
pCMV-Myc-chBRD2(1–179) | AGGAATTCAAATGCTGCAGAATGTGAATCC | TCCTCGAGTTACATTTGGGCCACCTTCTGCA | EcoRI/XhoI |
pCMV-Myc-chBRD2(1–447) | AGGAATTCAAATGCTGCAGAATGTGAATCC | GGCTCGAGTTAGTAGCTGAACTCAAAGACATCC | EcoRI/XhoI |
pCMV-Myc-chBRD2(1–510) | AGGAATTCAAATGCTGCAGAATGTGAATCC | TCCTCGAGTTAGTCAGAGCTCTCCTCGCTCTCT | EcoRI/XhoI |
pCMV-Myc-chBRD2(511–779) | GAGAATTCTCTCGGAAGAGGAACGTGCCAACC | TGCTCGAGTTAACCCGAATCCGAGTCGC | EcoRI/XhoI |
pCMV-Myc-chBRD2(619–683) | AGGAATTCAACCGATGACGTACGATGAGAAG | TTCTCGAGTTAGGACAGCACGTAGCGCTCCAG | EcoRI/XhoI |
pCMV-Myc-chBRD2/ET | AGGAATTCAACCGATGACGTACGATGAGAAG | TTCTCGAGTTAGGACAGCACGTAGCGCTCCAG | EcoRI/XhoI |
pCMV-Myc-huBRD2/ET | AGGAATTCAACCCATGAGTTACGATGAGAAG | TTCTCGAGTTAGGACAGCACGTAGCGCTCCAG | EcoRI/XhoI |
pCMV-HA-M(Δ264-313) | TTGAATTCGGATGGACTCATCCAGGAC | CTGGCTCCAGAGTATTCTCCTTATCTTTTCCTC | EcoRI |
GAAAAGATAAGGAGAATACTCTGGAGCCAGACC | GCACTCGAGTTATTTCCTGAAAGG | XhoI | |
pEGFP-M(Δ264-313) | TCGGAATTCAATGGACTCATCCAGGAC | CTGGCTCCAGAGTATTCTCCTTATCTTTTCCTC | EcoRI |
GAAAAGATAAGGAGAATACTCTGGAGCCAGACC | GCAGTCGACTTATTTCCTGAAAGG | SalI | |
pCMV-Myc-chBRD2(Δ619-683) | AGGAATTCAAATGCTGCAGAATGTGAATCC | TTTCTTCCGCAGGCATTTGCTCTCCTCTTCCTC | EcoRI |
GAAGAGGAGAGCAAATGCCTGCGGAAGAAACCC | TGCTCGAGTTAACCCGAATCCGAGTCGC | XhoI |