Skip to main content

Table 1 Primers used for the construction of recombinant expression plasmids

From: Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Recombinant plasmid

Sense primer (5′ → 3′)

Anti-sense primer (5′ → 3′)

Restriction sitesa

pEGFP-M

TCGGAATTCAATGGACTCATCCAGGAC

GCAGTCGACTTATTTCCTGAAAGG

EcoRI/SalI

pCMV-HA-M

TTGAATTCGGATGGACTCATCCAGGAC

GCACTCGAGTTATTTCCTGAAAGG

EcoRI/XhoI

pGEX-6p-M

TGCGAATTCATGGACTCATCCAGGAC

GGCACTCGAGTTATTTCCTGAAAGG

EcoRI/XhoI

pDsRed-chBRD2

CAGCTCGAGCTATGCTGCAGAATGTGAATCC

TGGGATCCTTAACCCGAATCCGAGTCGCT

XhoI/BamHI

pCMV-Myc-chBRD2

AGGAATTCAAATGCTGCAGAATGTGAATCC

TGCTCGAGTTAACCCGAATCCGAGTCGC

EcoRI/XhoI

pET-32a-chBRD2

CAGGAATTCATGCTGCAGAATGTGAATCC

TGCTCGAGTTAACCCGAATCCGAGTCGC

EcoRI/XhoI

pCMV-HA-M(1–178)

TTGAATTCGGATGGACTCATCCAGGAC

GACTCGAGTTACAAGGAGACAAAATTCAC

EcoRI/XhoI

pCMV-HA-M(1–245)

TTGAATTCGGATGGACTCATCCAGGAC

AACTCGAGTTATACAGTGGACATAAGCCC

EcoRI/XhoI

pCMV-HA-M(1–263)

TTGAATTCGGATGGACTCATCCAGGAC

GACTCGAGTTATCTCCTTATCTTTTCCTC

EcoRI/XhoI

pCMV-HA-M(264–364)

GAGAATTCGGCTCAATCTATCTGTTGG

GCACTCGAGTTATTTCCTGAAAGG

EcoRI/XhoI

pCMV-HA-M(314–364)

GAGAATTCGGATACTCTGGAGCCAGACC

GCACTCGAGTTATTTCCTGAAAGG

EcoRI/XhoI

pCMV-HA-M(264–313)

GAGAATTCGGCTCAATCTATCTGTTGGG

CTCTCGAGTTACTTGGCAACCTGGGGAGA

EcoRI/XhoI

pCMV-Myc-chBRD2(1–179)

AGGAATTCAAATGCTGCAGAATGTGAATCC

TCCTCGAGTTACATTTGGGCCACCTTCTGCA

EcoRI/XhoI

pCMV-Myc-chBRD2(1–447)

AGGAATTCAAATGCTGCAGAATGTGAATCC

GGCTCGAGTTAGTAGCTGAACTCAAAGACATCC

EcoRI/XhoI

pCMV-Myc-chBRD2(1–510)

AGGAATTCAAATGCTGCAGAATGTGAATCC

TCCTCGAGTTAGTCAGAGCTCTCCTCGCTCTCT

EcoRI/XhoI

pCMV-Myc-chBRD2(511–779)

GAGAATTCTCTCGGAAGAGGAACGTGCCAACC

TGCTCGAGTTAACCCGAATCCGAGTCGC

EcoRI/XhoI

pCMV-Myc-chBRD2(619–683)

AGGAATTCAACCGATGACGTACGATGAGAAG

TTCTCGAGTTAGGACAGCACGTAGCGCTCCAG

EcoRI/XhoI

pCMV-Myc-chBRD2/ET

AGGAATTCAACCGATGACGTACGATGAGAAG

TTCTCGAGTTAGGACAGCACGTAGCGCTCCAG

EcoRI/XhoI

pCMV-Myc-huBRD2/ET

AGGAATTCAACCCATGAGTTACGATGAGAAG

TTCTCGAGTTAGGACAGCACGTAGCGCTCCAG

EcoRI/XhoI

pCMV-HA-M(Δ264-313)

TTGAATTCGGATGGACTCATCCAGGAC

CTGGCTCCAGAGTATTCTCCTTATCTTTTCCTC

EcoRI

GAAAAGATAAGGAGAATACTCTGGAGCCAGACC

GCACTCGAGTTATTTCCTGAAAGG

XhoI

pEGFP-M(Δ264-313)

TCGGAATTCAATGGACTCATCCAGGAC

CTGGCTCCAGAGTATTCTCCTTATCTTTTCCTC

EcoRI

GAAAAGATAAGGAGAATACTCTGGAGCCAGACC

GCAGTCGACTTATTTCCTGAAAGG

SalI

pCMV-Myc-chBRD2(Δ619-683)

AGGAATTCAAATGCTGCAGAATGTGAATCC

TTTCTTCCGCAGGCATTTGCTCTCCTCTTCCTC

EcoRI

GAAGAGGAGAGCAAATGCCTGCGGAAGAAACCC

TGCTCGAGTTAACCCGAATCCGAGTCGC

XhoI

  1. aRestriction sites are given in italics and underlined.