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Figure 3 | Veterinary Research

Figure 3

From: Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Figure 3

Conservation analysis of the BRD2/ET domain from different species. A The complete coding sequences of the BRD2 gene from humans and other selected species were retrieved from the GenBank database. The amino acid sequences of the BRD2 protein (huBRD2 as the template) were analysed using the Clustal W multiple alignment algorithm in the MegAlign program of the DNASTAR Lasergene package. The different amino acids were marked in boxes and asterisks. B The three-dimensional crystal structures of the huBRD2/ET domain and chBRD2/ET domain (PDB accession no. 6CUI) were analysed using PyMOL. The different amino acids, including S in huBRD2/ET and T in chBRD2 and A in huBRD2/ET and S in chBRD2/ET, were marked in red and blue, respectively. C Identification of the interaction between the chBRD2/ET or huBRD2/ET domain and NDV M protein. Upper panel, schematic representation of the chBRD2/ET domain and huBRD2/ET domain. Lower panel, the combined plasmids were co-transfected into DF-1 cells, and 36 h after transfection, the interaction between HA-M and Myc-chBRD2/ET or Myc-huBRD2/ET was identified by co-IP assay.

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