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Figure 2 | Veterinary Research

Figure 2

From: Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Figure 2

Characterization of the binding domains between the NDV M protein and chBRD2 protein. A Mapping the binding domain of the NDV M protein for the chBRD2 protein. Plasmids expressing HA-tagged M or its truncation mutants and the chBRD2 protein were co-transfected into DF-1 cells. Thirty-six hours after transfection, the interactions between HA-tagged M or its truncation mutants and the chBRD2 protein were examined by co-IP assay. Upper panel, schematic representation of the full-length and truncation mutants of the HA-tagged M protein. Lower panel, HA-tagged M or its truncation mutants (Input) interacting with Myc-chBRD2 was detected by western blot. B Mapping the binding domain of the chBRD2 protein for the NDV M protein. Upper panel, schematic representation of the full-length and truncation mutants of the Myc-tagged chBRD2 protein. Lower panel, Myc-tagged chBRD2 or its truncation mutants (Input) interacting with HA-M was detected by western blot.

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