Figure 4

Effect of Deferiprone on the bacterial burden in challenged fish anterior kidney and plasma from treated fish on bacterial growth. A Relative P. salmonis load (arbitrary units, AU) in the anterior kidney from untreated (DFP −, white circles) or treated (DFP + , blue circles) fish during the course of the experiment at different S0 to S5 sampling points (0, 4, 8, 16, 23, 30 days post-challenge (dpc), respectively). Each point represents the mean ± SD of 5 fish measured in at least two independent experiments. Two-way ANOVA and subsequent Bonferroni comparison test relative to control (DFP −) was performed. Asterisks show statistically significant differences denote: **p-value < 0.01, ****p-value < 0.0001. B Bacterial growth of P. salmonis in SRS broth replacing fetal bovine serum with plasma from unchallenged and untreated (DFP −, white box) or treated (DFP + , blue box) fish and quantified at the stationary phase of bacterial growth (5 days post-inoculation). Data show box and whisker plot and solid horizontal lines indicate the median and the 25th 75th interquartile range level of at least five independent experiments. Unpaired t-test was performed between groups; ns = statistically non-significant.