Figure 2

Effect of Deferiprone in SHK-1 cell viability infected with Piscirickettsia salmonis. A Representative microphotograph of SHK-1 monolayer exposed (DFP +) or not (DFP −) to DFP (25 μM) in panoptic staining. Upper panels show uninfected cells and lower panels P. salmonis infected cells after 10 days of infection and DFP treatment, Bar = 10 μm. B The ratio of cytopathic (CPE) on total cells (left axis) in grey bars and area of P. salmonis containing vacuoles (PCV) represented by red circles in SHK-1 DFP treated/untreated and infected/uninfected cells. C Cell viability percentage (left axis) in grey bars and relative P. salmonis load (arbitrary units, AU) (right axis) represented by red circles, in SHK-1 DFP treated/untreated and infected/uninfected cells. For A and C, data represent mean ± SD of 10 observations measured in at least three independent experiments. One-way ANOVA and Tukey multiple comparisons between all treatments were performed; different letters represent significant differences (p-value < 0.05; capital letters for left axis and lowercase letters for right axis comparison).