Table 1 The primer sequences for RT- and QRT-PCR.
From: PRV UL13 inhibits cGAS–STING-mediated IFN-β production by phosphorylating IRF3
Name | Sequence |
|---|---|
PigIFNB1-F | 5′-TTGGCATGTCAGAAGCTCCT-3′ |
PigIFNB1-R | 5′-CTGGAATTGTGGTGGTTGCA-3′ |
PigGAPDH-F | 5′-CCTTCATTGACCTCCACTACA-3′ |
PigGAPDH-R | 5′-GATGGCCTTTCCATTGATGAC-3′ |
CanineIFNB1-F | 5′-ACTTCACCTGGGACAACAGG-3′ |
CanineIFNB1-R | 5′-GCTGTACTCCTTGGCCTTCA-3′ |
CanineGAPDH-F | 5′-GGCTGAGAACGGGAAACTTG-3′ |
CanineGAPDH-R | 5′-TCACCCCATTTGATGTTGGC-3′ |
HumanIFNB1-F | 5′-TTCACCAGGGGAAAACTCAT-3′ |
HumanIFNB1-R | 5′-TCCTTGGCCTTCAGGTAATG-3′ |
HumanGAPDH-F | 5′-CCACCCAGAAGACTGTGGAT-3′ |
HumanGAPDH-R | 5′-TTCAGCTCAGGGATGACCTT-3′ |
ISG15-F | 5′-ACTGCATGATGGCATCGGAC-3′ |
ISG15-R | 5′-CAGAACTGGTCAGCTTGCAC-3′ |
ISG20-F | 5′-CAGGATTCCCGGCTTGAAGT-3′ |
ISG20-R | 5′’-CTGGCATCTTCCACCGAGTT-3′ |
ISG54-F | 5′-GCACAGCAATCATGAGTGAGAC-3′ |
ISG54-R | 5′-TTTCCTCCACACTTGAGCCG-3′ |
ISG56-F | 5′-GACCTACGTCTTCCGACACG-3′ |
ISG56-R | 5′-CTTCTGCTTTGCTGTGGTCG-3′ |
RNase L-F | 5′-AAGCGCCATAACAACCCTCA-3′ |
RNase L-R | 5′-GCATGTTCACGTCTGCTCCA-3′ |
Viperin-F | 5′-GCACCTGGACTCTGATTGCT-3′ |
Viperin-R | 5′-TTGGGCAAAACAGCTCATGC-3′ |
MxA-F | 5′-GATCCGGCTCCACTTCCAAA-3′ |
MxA-R | 5′-CTCTTGTCGCTGGTGTCACT-3′ |
PKR-F | 5′-GCACTTCTAGCCATCTGGTCA-3′ |
PKR-R | 5′-GATGTGCTCGTTGTGGGAGA-3′ |
Actin-F | 5′-TGCTGTCCCTGTACGCCTCTG-3′ |
Actin-R | 5′-ATGTCCCGCACGATCTCCC-3′ |