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Figure 6 | Veterinary Research

Figure 6

From: PRV UL13 inhibits cGAS–STING-mediated IFN-β production by phosphorylating IRF3

Figure 6

UL13 suppresses the expression of ISGs induced by cGAS–STING or poly(I:C). A PK15 cells were transfected with a mixture of pcDNA4-HA-cGAS (50 ng) and pcDNA3-Flag-STING (20 ng), together with pcDNA3-Flag or pcDNA3-Flag-UL13 (200 ng), for 30 h. The total amount of DNA was normalized to the corresponding empty vectors. The mRNA expression levels of ISGs were determined by semi-quantitative RT-PCR. B The experiment was performed as in (A), except that the PK15 cells were transfected with pcDNA3-Flag or pcDNA3-Flag-UL13 (500 ng) for 24 h, and then either treated with poly(I:C) (10 μg/mL) for 9 h or not.

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