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Figure 3 | Veterinary Research

Figure 3

From: PRV UL13 inhibits cGAS–STING-mediated IFN-β production by phosphorylating IRF3

Figure 3

PRV UL13 inhibits poly(I:C)- or VSV-induced IFN-β transactivation. A, C PK15 cells were transfected with IFN-β-Luc (200 ng), pCMV-RL (2 ng) mixed with pcDNA3-Flag (200 ng), or pcDNA3-Flag-UL13 (200 ng) for 24 h; the cells were then treated with poly(I:C) (10 μg/mL) in (A) or infected with VSV (1 MOI) in (C) for 9 h. The fold activation of luciferase activity is the product of the luciferase activity induced by poly(I:C) with or without PRV UL13 divided by that induced by the empty vector. B, D The dual-luciferase reporter assays were performed as in (A, C), except that IRF3-Luc was used instead of IFN-β-Luc.

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