Skip to main content
Figure 2 | Veterinary Research

Figure 2

From: PRV UL13 inhibits cGAS–STING-mediated IFN-β production by phosphorylating IRF3

Figure 2

UL13 suppresses IFN-β transactivation by the cGAS–STING effectors, TBK1, and IRF3. A, G PK15 cells were transfected with IFN-β-Luc (200 ng) in (A) or IRF3-Luc (200 ng) in (G), pCMV-RL (2 ng) mixed with pcDNA4-HA-cGAS (50 ng), pcDNA3-Flag-STING (20 ng), pcDNA3-Flag (200 ng) or pcDNA3-Flag-UL13 (200 ng). The cells were collected 30 h post-transfection and then analysed for luciferase activity. The fold activation of luciferase activity is calculated as the luciferase activity induced by cGAS–STING with or without PRV UL13, divided by that induced by the empty vector. B, H Dual-luciferase reporter assays were performed as in (A, G), except that pcDNA4-HA-TBK1 (250 ng) was used instead of pcDNA4-HA-cGAS (50 ng) and pcDNA3-Flag-STING (20 ng). C, I The dual-luciferase reporter assay was performed as in (A, D) except that pcDNA4-HA-IRF3/5D (250 ng) was used instead of pcDNA4-HA-cGAS (50 ng) and pcDNA3-Flag-STING (20 ng). DF were conducted as in (AC), except that transfection was performed without FN-β-Luc and pCMV-RL. The mRNA expression of IFN-β was then analysed by QRT-PCR.

Back to article page