Figure 3

Monocytes from infected animals exhibit higher phagocytic activity than monocytes from control animals. Mononuclear cells were isolated from the blood of infected and uninfected animals via density gradient centrifugation using Histopaque-1077 and cultured in the presence of yellow green (YG) fluorescent beads. A duplicate plate was stored at 4 °C as a negative control for phagocytosis. A Gating strategy used to analyse mononuclear cells. We gated out debris, doublets, and dead cells (7-AAD-positive cells), and evaluated the percentage of cells that were MRC1L-B⁺YG⁺ double positive. B Quantitative data regarding the percentage of monocytes that phagocytized the beads at 41 °C or 4 °C (control). The data are presented as the percentage of 7-AAD-negative cells. Each dot represents an animal. A total of 20,000 events per sample were acquired using a FACSMelody flow cytometer. The results are expressed as the mean ± standard deviation values. Significant differences are indicated by **p = 0.0079.