Figure 2

Evaluation of monocytes and surface expression levels of MHC-II and MHC-I molecules on different days post infection. Mononuclear cells were isolated from the blood of infected and uninfected animals at 4, 9, 12, and 25 days post infection via density gradient centrifugation using Histopaque-1077. A MHC-II surface expression on monocytes and MHC-II⁺MRC1L-B⁻ cells was evaluated using the analysis protocol shown in Figure 1A. Each dot represents an animal. Significant differences are indicated by *p = 0.0159. B Each dot represents an experimental group (n = 5) evaluated on different days post infection. Significant differences are indicated by *p = 00,317. C Histogram showing the different levels of MHC-I expression on monocytes from infected and uninfected animals. D MHC-I surface expression in the monocyte population was evaluated on different days post infection. The results are expressed as the mean ± standard deviation values. A total of 30,000 events per sample were acquired using a Gallios flow cytometer.