Figure 9

rTsP-mediated promotion and anti-rTsP serum-mediated inhibition of the larval invasion of excised intestine. Two hundred IIL were pre-incubated with rTsP, anti-rTsP serum, infection serum, pre-immune serum, BSA, or PBS; subsequently injected into the excised mouse gut lumen; and maintained in Tyrode’s solution at 37 °C for 2 h. The enteral segment was opened, and the enteral mucosa was compressed and observed under microscopy. A Non-invaded larvae showed a spiral coil-like shape within the enteral lumen. B Invaded larvae were inside the enteral mucosa. C Non-invaded larvae in the caecum were used as a negative control. D and E The inhibitory and promotive effects of the pre-incubation of larvae with anti-rTsP serum (D) or rTsP (E) on larval invasion of the enteral mucosa are shown. *P < 0.01 compared to pre-immune serum or BSA. Scale bars: 100 μm.