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Figure 6 | Veterinary Research

Figure 6

From: Molecular cloning and characterization of a novel peptidase from Trichinella spiralis and protective immunity elicited by the peptidase in BALB/c mice

Figure 6

The specific binding of rTsP with IECs and the cellular localization of rTsP. A IIFA was used to identify the specific binding of rTsP with IECs. IECs or C2C12 cells were pre-incubated with rTsP, IIL ES antigens or PBS. After blocking and washing, the IECs and C2C12 cells were incubated with anti-rTsP serum, infection serum or pre-immune serum, followed by incubation with FITC-conjugated anti-mouse IgG. Cell nuclei were re-dyed blue using DAPI. Scale bars: 50 μm. B The cellular localization of rTsP in IECs was determined by confocal microscopy. The IECs were first pre-incubated with rTsP; probed with anti-rTsP serum, infection serum or pre-immune serum; and finally stained using FITC-conjugated anti-mouse IgG. Scale bars: 5 μm.

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