Figure 4

Expression and immunolocalization of TsP in T. spiralis at various stages by IIFA using anti-rTsP serum. A Expression of TsP at the epicuticle of T. spiralis at various stages was determined by IIFA. Intact whole worms were probed by anti-rTsP serum, and immunostaining was observed on the cuticle of worms at various stages. a ML; b 6 h IIL; c 24-h IIL; d 3-day-old AWs; e 6-day-old AWs; f NBL. g ML probed using T. spiralis-infected serum were used as a positive control. ML probed with pre-immune serum (h) and PBS (i) were used as a negative control. Scale bars = 50 μm. B Immunolocalization of TsP in worm cross-sections from T. spiralis at various stages was determined by IIFA using anti-rTsP serum. Immunostaining was observed at the cuticle of ML, IIL, and intrauterine embryos of female AWs. No immunostaining on worm cross-sections was observed when pre-immune serum was used as a negative control. ML and IIL scale bars: 50 μm. AW scale bars: 100 μm.