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Figure 2 | Veterinary Research

Figure 2

From: Molecular cloning and characterization of a novel peptidase from Trichinella spiralis and protective immunity elicited by the peptidase in BALB/c mice

Figure 2

SDS-PAGE and western blot analysis of rTsP. A SDS-PAGE analysis of lysates of BL21 bacteria transfected with pQE-80L/TsP before and after induction. Lane M: protein marker; lane 1: lysate of BL21 bacteria transfected with pQE-80L/TsP prior to induction; lane 2: lysate of BL21 bacteria transfected with pQE-80L/TsP following induction; lane 3: purified rTsP. B SDS-PAGE analysis of rTsP. Lane M: protein marker; lane 1: ML soluble proteins; lane 2: ML ES proteins; lane 3: purified rTsP. C Western blot analysis of rTsP antigenicity. ML soluble proteins (lane 1), ML ES proteins (lane 2) and rTsP (lane 3) were identified by T. spiralis-infected sera. Native TsP among ML soluble proteins (lane 4) and ES proteins (lane 5), and rTsP (lane 6) was recognized by anti-rTsP serum. ML soluble proteins (lane 7), ES proteins (lane 8), and rTsP (lane 9) were not recognized by normal murine serum.

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