Figure 7

Lymphocyte proliferation assay and analysis of IFN-γ and IL-4 by ELISA. A Lymphocyte proliferation assay was done using PBMCs of swine as described in “Materials and methods” section. The optical density (OD) was determined at 490 nm, and the stimulation index (SI) was calculated as follows: SI = mean OD of PCV2 stimulated cells/mean OD of unstimulated cells. B Analysis of IFN-γ and IL-4 by ELISA was performed using the IFN-γ and IL-4 ELISA Kit. The value of OD₄₀₅ nm was measured with an ELISA reader. Data represent the mean ± SD. A P value less than 0.05 was considered significant.