Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap(d41) protein and analysis of its immunogenicity in mice and swine

Figure 4

Optimum conditions for production of the PCV2 Cap(d41) protein. Effects of different infection times, MOI, and cell numbers for expressing the Cap(d41) protein were tested. A, B Cell numbers (107) of Sf-9 cells were infected with MOI of 10 (A) or infected with different MOI (B) for 4 days. Cell numbers (107) of Sf-9 cells were infected with different recombinant baculoviruses as indicated. The Sf-9 cell alone and CE recombinant baculoviruses were used as the negative control. Signals in all western blots were quantified using Image J software. β-actin was used as an internal control for normalization. Relative abundance (%) are shown. The results were calculated from the data shown in the upper portion of each panel. All data shown represent the mean ± SD calculated from three independent experiments. A student t-test was conducted for analysis and a value of P < 0.05 was considered statistically significant. C Expression levels of the PCV2 Cap(d41) protein in different cell numbers infected with recombinant baculovirus at an MOI of 10 for 4 days was examined. Signals in all western blots were quantified using Image J software. β-actin was used as an internal control for normalization. Relative abundance (%) are shown. The results were calculated from the data shown in the left portion of each panel. All data shown represent the mean ± SD calculated from three independent experiments. A student t-test was conducted for analysis and a value of P < 0.05 was considered statistically significant. D The immune effect of different doses of Cap(d41) protein and evaluation of the effective dose. Different cell numbers (105, 106 and 107) of Sf-9 cells were infected with recombinant baculoviruses BacSC-Cap(d41) and BacDD-4Cap(d41), respectively, at an MOI of 10 for 3 days, respectively and cell lysates were collected to immunize mice. After two vaccinations, antibody titers were analyzed by ELISA. Data represent the mean ± SD. A p value less than 0.05 was considered significant.

Back to article page