Figure 4
Immunohistochemistry (×200) to differentiate the Hyaluronidase expression between the two groups. Immunohistochemistry was performed in the larvae: Anti-hyaluronidase antibody was used to stain (brown) the tissues having hyaluronidase. Harris hematoxylin (blue) was used as counterstain. Brown parts show the presence of hyaluronidase, and parts in blue reflect its absence. In negative control group (A) higher expression of Hyaluronidase can be seen. The HAase-siRNA group (B) shows a significant lacking of hyaluronidase expression.