Figure 2

CD45 expression on A and B heterophils and C and D monocytes. Chickens were uninfected, or experimentally infected with ER on day 0, “naïve” and “vaccinated” groups. Blood samples were collected at the indicated days. In A and C expression is shown as median fluorescence intensity (MFI) in the heterophil and monocyte gates, respectively, as defined in Additional file 2, and results are shown as group mean values ± 95% CI where non-overlapping CI indicate statistically significant differences. On days –3 and 15 n = 13/group, on days 1, 5 and 8 n = 7/group and on days 3 and 11 n = 6/group. In B CD45 expression on heterophils on day 1 after ER infection is shown as fluorescence intensity for one representative uninfected, “naïve” and “vaccinated” chicken, respectively. In D CD45 expression on monocytes on day 3 after ER infection is shown as fluorescence intensity for one representative uninfected, “naïve” and “vaccinated” chicken, respectively. Histograms in B and D were compiled using the FlowJo™ software package. For details see "Materials and methods" section.