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Figure 6 | Veterinary Research

Figure 6

From: NLRP3 played a role in Trichinella spiralis-triggered Th2 and regulatory T cells response

Figure 6

Cytokine levels of CD4+ T cells by MLES –treated DCs in vitro. For T helper-related cytokines expression, after coculture of DCs (1 × 105/well) and CD4+ T cells (1 × 106/well) for 72 h with OVA (1 mg/mL), then cells were incubated with 10 mg/mL Brefeldin A, 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 750 ng/mL Ionomycin for 6 h at 37 °C. A Percent of CD4+ IFN-γ+ T cells and CD4+ IL-4+ T cells were shown. B Percent of CD4+ IL-4+ T cells was shown. (B) Percent of CD4+ IL-10+ T cells was shown. C Percent of CD4+ TGF-β+ T cells was shown. D Data are shown as the mean ± SD (three independent experiments) of each group (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 as indicated by line (one-way ANOVA with Tukey’s posttest). These figures are representative of three independent experiments.

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