Figure 2

NLRP3 played a role in T. spiralis -triggered immune response in vivo. WT and NLRP3^−/− mice were injected intraperitoneally (i.p.) with phosphate-buffered saline (PBS) or MLES (100 μg) twice at 7 days intervals. Spleen cells were isolated 7 days after second injection. A IFN-γ, IL-4, IL-10 and TGF-β production was measured by ELISA. B Treg populations induced by MLES were measured. CD4⁺CD25⁺Foxp3⁺ T cells were determined by flow cytometry. The data represent the means ± standard deviations (SD) of each group (n = 6) of the results from three individual experiments *p < 0.05, **p < 0.01, and ***p < 0.001 as indicated by the line (one-way ANOVA with Tukey’s post-test). These figures are representative of three independent experiments.