|
Group
|
NE
|
Gelatinolytic enzyme, approximate MW
|
|---|
|
~ 40–45 kDa
|
~ 50–60 kDa
|
~ 60–70 kDa
|
~ 75–85 kDa
|
|---|
|
Control (n = 5)
|
No
|
1.10 ± 0.054A
|
1.19 ± 0.072
|
1.03 ± 0.039
|
1.09 ± 0.077
|
|
Type A (n = 5)
|
No
|
0.81 ± 0.083B
|
1.10 ± 0.15
|
1.05 ± 0.14
|
1.09 ± 0.18
|
|
Type G (n = 5)
|
No
|
0.74 ± 0.051B
|
1.01 ± 0.086
|
0.93 ± 0.063
|
0.89 ± 0.087
|
|
Type G (n = 9)
|
Yes
|
0.65 ± 0.030
|
0.98 ± 0.045
|
0.86 ± 0.051
|
0.74 ± 0.045
|
- Control: Eimeria infected birds, Type A and Type G: Eimeria infected birds challenged with respectively a commensal C. perfringens type A strain (JIR4857) or pathogenic C. perfringens type G strain (CP56). NE: birds without (No) or with (Yes) necrotic enteritis. Gelatinolytic activity of the digested spots is expressed in arbitrary units = \(\frac{100}{{OD/{\text{mm}}^{2} }}\). Data represents mean ± SE. One-way ANOVA followed by Tukey’s multiple comparisons test was used to compare the means of the NE = No groups. Values within the same column with different superscripts differ significantly (p < 0.05).
