Group
|
Segment
|
Lesion scorea
|
NE positiveb
|
Average lesion scorec
|
---|
0
|
2
|
3
|
4
|
5
|
6
|
---|
Control
|
Duodenum
|
26
|
0
|
0
|
0
|
0
|
0
|
0% (0/26)
|
–
|
Jejunum
|
26
|
0
|
0
|
0
|
0
|
0
|
0% (0/26)
|
–
|
Ileum
|
26
|
0
|
0
|
0
|
0
|
0
|
0% (0/26)
|
–
|
|
Overall
| | | | | | |
0% (0/26)
|
–
|
Type A (JIR 4857)
|
Duodenum
|
27
|
0
|
0
|
0
|
0
|
0
|
0% (0/27)
|
–
|
Jejunum
|
27
|
0
|
0
|
0
|
0
|
0
|
0% (0/27)
|
–
|
Ileum
|
27
|
0
|
0
|
0
|
0
|
0
|
0% (0/27)
|
–
|
|
Overall
| | | | | | |
0% (0/27)
|
–
|
Type G (CP56)
|
Duodenum
|
85
|
11
|
6
|
4
|
1
|
0
|
20.56% (22/107)A
|
2.77C
|
Jejunum
|
19
|
7
|
15
|
46
|
18
|
2
|
82.24% (88/107)B
|
3.92D
|
Ileum
|
89
|
6
|
5
|
6
|
0
|
1
|
16.82% (18/107)A
|
3.17C
|
|
Overall
| | | | | | |
85.05% (91/107)
|
3.91
|
- All birds were challenged with a ten-fold dose of a live attenuated Eimeria vaccine at day 14 and 16 to induce a predisposing coccidiosis infection. On day 18, 19 and 20, birds were challenged with either a pathogenic C. perfringens type G strains (CP56), a commensal C. perfringens type A strain (JIR4857) or sterile bacterial culture medium (control).
- aNE lesion scoring of the small intestine was performed as previously described by Keyburn et al. [28].
- bNE positive = lesion score ≥ 2.
- cAverage lesion score of NE positive birds. Data represents mean ± SE.
- dMeans within the same column with different superscripts differ significantly (p < 0.05); A, B binary logistic regression analysis or C, D one-way ANOVA followed by Tukey’s multiple comparisons test.