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Table 1 Most relevant bacterial strains used in this work.

From: Disruption of pyruvate phosphate dikinase in Brucella ovis PA CO2-dependent and independent strains generates attenuation in the mouse model

Brucella ovis strainsa

Main characteristics

Source

B. ovis PA

Virulent parental strain; CO2-dependent

BCCN (76-250)

Bov::CA

B. ovis PA carrying Tn7Ba2308WCAIBa2308WCAII; CO2-independent

This work

BovΔppdK

Derived from B. ovis PA; ppdK deletion; CO2-dependent

This work

BovΔppdK pAZI-19

BovΔppdK complemented in trans with ppdK gene cloned into pRH001; CO2-dependent

This work

Bov::CAΔppdK

Derived from Bov::CA; ppdK deletion; CO2-independent

This work

Bov::CAΔppdK pAZI-19

Bov::CAΔppdK complemented in trans with ppdK gene cloned into pRH001; CO2-independent

This work

  1. aThe B. ovis recombinant strains derive from B. ovis PA, which was obtained from BCCN (Brucella Culture Collection Nouzilly, Institut National de la Recherche Agronomique, Nouzilly, France).