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Figure  3 | Veterinary Research

Figure  3

From: Disruption of pyruvate phosphate dikinase in Brucella ovis PA CO2-dependent and independent strains generates attenuation in the mouse model

Figure  3

Spleen colonization kinetics of strains derived from CO2-dependent (A–C) and CO2-independent (D) B. ovis PA. In a first experiment (A, B), animals were inoculated with 106 CFU (A) or 108 CFU (B) of B. ovis PA or the ppdK mutant. The results are expressed as mean ± SD of the log CFU/spleen (n = 5) detected for each strain at weeks 3 and 11 p.i. In a second experiment (C, D), mice were inoculated with 108 CFU of the CO2-dependent and CO2-independent strains and bacterial CFU were determined in spleen at weeks 1, 3, 5, 7 and 11 p.i. The results are expressed as mean ± SD of the log CFU/spleen (n = 5) detected for each strain. For each time point, statistically significant differences (P < 0.01), when compared to mice inoculated with the corresponding parental strain, are marked with asterisks (***P ≤ 0.0005).

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