Figure 2

Diversity analysis of colonic microbiota. A Diversity analysis of colonic microbiota. The rarefaction abundance curves, Shannon index, and rank abundance curves were used to assess the colonic microflora diversity at 97% similarity in 4 groups (n = 6). B Difference analysis of alpha diversity index (Sobs, Shannon, and Ace index) between groups. Alpha diversity was illustrated by number of observed OTU using a one-way ANOVA. *P < 0.05. C Comparative analysis of colonic microbiota. Beta diversity was illustrated by Principal coordinate analysis (PCoA) of the overall microbial composition (Distance Algorithm: unweighted unifrac, ANOSIM R: 0.591, P = 0.001). Partial Least Squares Discriminant Analysis (PLS-DA) was used to represent sample grouping analysis results (Distance Algorithm: unweighted unifrac). D Heatmap analysis showed the relative abundance of the bacterial at the phylum level in four groups. E Differences in the relative abundance of genera from the 4 groups are shown using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. F Heatmap analysis showed the relative abundance of the top 50 genera in four groups. G Differences in relative abundance of genera from the 4 groups are shown using one-way ANOVA. All these data were presented as mean ± SEM. *P < 0.05, **P < 0.01.