Figure 4
From: The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis
Analysis ofHAPS_0849by activity assay and gene complementation withE. coli pgm. A Expression and purification of HAPS_0849 in E. coli BL21 (DE3). Lane M, protein molecular marker; lane 1, purified and diluted HAPS_0849. B PGM activity assay described in “Materials and methods” section. G1P was used as the substrate; the reaction started only with 6-phosphoglucose dehydrogenase (G6PDH) was set as the negative control (NC), OcPGM was from rabbit muscle as positive control. In comparison with the NC, HAPS_0849 exhibited significant PGM activities in the enzyme-linked reaction (*P < 0.05). Bars represent the mean ± standard error of three independent experiments. C LOS profiles of the G. parasuis SC096, ΔHAPS_0849 and Ecpgm-expressing strains. Lane M, protein molecular marker; lane 1, wild-type; lane 2, ΔHAPS_0849 mutant; lane 3, ΔHAPS_0849-ompP5::Ecpgm.