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Table 1 Experimental design of functional analysis

From: Sub-lethal doses of albendazole induce drug metabolizing enzymes and increase albendazole deactivation in Haemonchus contortus adults

 

Pre-incubation 18 h

 

Incubation 12 h

Control

Affected

Control

Affected

Blanks

DMSO 0.1%

0.01 µM ABZ

0.1 µM ABZ

1.0 µM ABZ

WASHING

DMSO 0.1%

DMSO 0.1%

1

DMSO 0.1%

0.01 µM ABZ

0.1 µM ABZ

1.0 µM ABZ

10 µM ABZ

10 µM ABZ

2

DMSO 0.1%

0.01 µM ABZ

0.1 µM ABZ

1.0 µM ABZ

10 µM ABZ

10 µM ABZ

3

DMSO 0.1%

0.01 µM ABZ

0.1 µM ABZ

1.0 µM ABZ

10 µM ABZ

10 µM ABZ

  1. Affected groups of helminths were pre-incubated with sub-lethal concentrations of ABZ (0.01, 0.1 and 1.0 µM) for 18 h. Control groups were pre-incubated with no ABZ. Both affected and control groups were followingly incubated with high ABZ concentration (10 µM) for 12 h except the blanks.